Biomolecular NMR Facility

Overview of Services

The Biomolecular NMR Facility is an interdepartmental research service unit located in the Biochemistry Department. High-field NMR spectroscopy is a powerful technique for the study of biomolecular structure and dynamics. The facility provides service for routine 1D and 2D NMR, and also provides consultation and collaborative assistance with the acquisition and analysis of multidimensional, multinuclear protein NMR spectra. The facility maintains four Bruker NMR spectrometers (Avance III 500 MHz, two Avance-III HD 600 MHz, and Avance-Neo 800 MHz) which are operated and maintained by Dr. Francis Peterson (Professor of Biochemistry and NMR facility manager) in a dedicated 2500 ft2 facility in the Health Research Center basement. Each NMR instrument is equipped with a ¹H/¹⁵N/¹³C cryoprobe for ultra-high sensitivity in biomolecular applications. The 500 MHz instrument also has 19F detection, and the 800 MHz cryoprobe is equipped with three-axis XYZ pulsed-field gradients. SampleJet robots for automated NMR acquisition of samples in 96-well sample racks are installed on the 600 and 800 MHz instruments. One 600 MHz instrument is configured with a 1.7mm ¹H/¹⁵N/¹³C cryoprobe (~60 µL sample size) and dedicated to chemical fragment screening and other automated multi-sample/high-throughput NMR applications. For long-term projects, the facility provides training for instrument operation and data analysis to investigators and research personnel. The facility operates on a fee-for-service basis and is open to faculty of the Medical College of Wisconsin and outside researchers. 

Leadership

Location and hours of operation

Links and Resources

1. https://www.mcw.edu/departments/biochemistry/research-facilities

Contacts