Rodent Model Resource (RMR)

Rodent Model Resource (RMR) at MCW with support of Versiti

(Formerly Mouse Transgenic Core)

The RMR provides access to the following services for rodent models

Custom production of novel transgenic, gene knockout, and gene knockin rat and mouse models
Management of rat model breeding colonies

Assists research staff with procedures involving production and maintenance of transgenic mouse and rat strains. The Core provides both sperm and embryo cryopreservation/recovery, IVF, ICSI, pronuclear microinjection of CRISPR and DNA/BAC constructs. We are also available for consultation involving colony breeding issues.

The laboratory is located in the vivarium of the Translational and Biomedical Research Center at MCW and is equipped to perform all procedures involving production and maintenance of transgenic or knock-out mice. Instrumentation includes 2 injection workstations (Nikon inverted phase contrast microscope with Hoffman modulation optics, temperature-controlled injection stage, air-suspended injection table, Eppendorf motor-driven injection system), microforge, needle puller, Nikon stereo zoom microscope, surgical microscopes and dewars for storage of cryopreserved mouse germplasm.

Leadership

Aron Geurts, PhD

Professor, Department of Physiology

Contacts

Name	Role	Phone	Email	Location
Jason Klotz		414-456-7580	jklotz@mcw.edu	BSB
Michael Grzybowski	Geurts Lab manager	414-955-7576	mgrzybowski@mcw.edu	BSB

Service list

Name	Description	Price
NRG/Jax mice	007799 - NRG Strain Details the RMR holds a small breeding colony of these mice for INTERNAL investigators and non-profit research use ONLY under the terms below. Offspring are available on as available basis. contact transgeniccore@mcw.edu for more informaiton. An academic institution may use the stock#007799, NOD.Cg-Rag1<tm1Mom> Il2rg<tm1Wjl>/SzJ, for academic research, usually under grant funding. Mice may not be transferred or used for commercial use. The mouse or progeny of any kind, or a modified version of the mouse may not be shipped to any party (e.g. a different institution, researcher or company etc.), outside of the institution without the prior written consent of Jackson. Any researcher using a Jackson mouse should be aware of the full and correct ID of the mouse model and that there may be specific restrictions on use of any strain.	Inquire
Strain maintenance (mice, cage days)	includes BRC per diem cost	Inquire

Search available services: View: by category alphabetically

Name	Description	Price
▼ ► Mouse Service Lines (1)
NRG/Jax mice	007799 - NRG Strain Details the RMR holds a small breeding colony of these mice for INTERNAL investigators and non-profit research use ONLY under the terms below. Offspring are available on as available basis. contact transgeniccore@mcw.edu for more informaiton. An academic institution may use the stock#007799, NOD.Cg-Rag1<tm1Mom> Il2rg<tm1Wjl>/SzJ, for academic research, usually under grant funding. Mice may not be transferred or used for commercial use. The mouse or progeny of any kind, or a modified version of the mouse may not be shipped to any party (e.g. a different institution, researcher or company etc.), outside of the institution without the prior written consent of Jackson. Any researcher using a Jackson mouse should be aware of the full and correct ID of the mouse model and that there may be specific restrictions on use of any strain.	Inquire
▼ ► Strain Maintenance (1)
Name	Description	Price
Strain maintenance (mice, cage days)	includes BRC per diem cost	Inquire