ALERT

ATTENTION ⚠️

On Friday June 14th, 2024 at 11:30PM (UTC), maintenance has been scheduled for iLab ERP and this will affect institutions utilizing the financial integration feature on iLab. Maintenance is planned for 15 minutes and during this time, there is a server downtime of no more than 10 minutes. iLab Sites will remain operational for daily workflow and access. Details and updates can be found on the Agilent Status Page

Cell Therapy Lab

Overview of Services

The Cell Therapy Lab shared resource is a state-of-the-art laboratory for the MCW and extramural community. The Cell Therapy Lab has established itself as a leader in the area of in-house manufacturing of novel cell therapies for early-phase clinical trials. It also provides the expertise and equipment needed for cutting-edge immune profiling of samples from clinical or preclinical studies. We aim to support researchers in everything from a single analysis to comprehensive correlative studies for multi-site clinical trials.

Key services include:
• Miltenyi CliniMACS Prodigy system for in-house manufacturing of clinical grade CAR-T cell and immunotherapy products
• Miltenyi CliniMACS system for automated clinical scale cell enrichment or depletion
• Berkeley Lights Lightning Optofluidic system for groundbreaking single-cell studies. The system performs simultaneous assessment of single-cell function and phenotype, with the capacity to perform on-chip single-cell transcriptomic, and to re-isolate cells of interest for additional studies
• Single-cell and bulk cytokine & biomarker multi-plex assays using the IsoPlexis and Luminex platforms
• Real-time cell cytotoxicity assays using the xCelligence RTCA system
• Assays of cell proliferation, ELISA, PCR (droplet digital system and standard PCR) and immunophenotyping using a MACS Quant 10 flow cytometer (8-colors)
• In addition to providing access to immune monitoring instruments, we also provide project and grant development assistance

Leadership

Tyce Kearl, MD, PhD Co-Director
Peiman Hematti, MD Co-Director

Location and hours of operation

Hours Location

Monday - Friday, 8am- 5pm 

9200 W. Wisconsin Ave.

Froedtert Hospital Pavilion (STE 302-324) and Clinical Cancer Center (Rm C5453)

Milwaukee, WI 53226

 

Contacts

Name Role Phone Email Location
Tyce Kearl, MD, PhD
Research Director
 
414-805-7420
 
tkearl@mcw.edu