Overview of CVRC Core
CVRC-iPSC Core Services
The Human Induced Pluripotent Stem Cell (iSPC) Program will be sunsetting on June 30, 2025. Beginning in FY26, a new human iPSC core is planned that includes services such as reprogramming, CRISPR k/i and k/o, single nucleotide editing, premade cell products, and differentiation. Please check back with the CVRC staff for additional details and updates at this time.
CVRC-Microscopy Core
All users must be members of the CVC. Contact Andrew Barr for guidance on becoming a CVC member.
$25/hr charge for use of the Nikon A1R, all other resources are free to use once trained.
Inverted confocal microscopes:
- Nikon A1R Laser Scanning Confocal ($25/hr)
- Training initiated & reserved via iLab
- 10-100x DIC objectives
- Excitation laser lines 405/488/561/640 nm
- Emission band passes (425-475/500-550/570-620/663-738)
- Galvano or fast resonant scanning
- Live cell imaging chamber: OKO lab boldline temp + CO2/O2 stage top incubator available
- LED light source
- NIS-Elements AR v.5.21.03
- Advanced research package capable of mult-dimensional image acquisition including multiple XY positions, z-series, multiple channels, time and large image stitching
- Nikon’s denoise.ai
- Photobleaching/photo-stimulation module available (Galvano = all lasers available for photobleaching/photostimulation; Resonance = only 405 nm laser available for photobleaching/photostimulation)
- Nikon C2+ Laser Scanning Confocal (no charge)
- Training initiated via iLab
- Reservation via shared outlook calendar
- 20-40x objectives mounted; 60x & 100x available as needed
- Excitation laser lines 405/488/561/640
- Manual 2 detector system allows simultaneous imaging of up to 3 channels (4 channels may be imaged sequentially):
- Position 1: 445/35 nm & 660 nm LP
- Position 2: 525/50 nm & 600/50 nm
- Live cell imaging chamber: OKO lab boldline temp + CO2/O2 stage top incubator available
- Metal halide light source
- NIS-Elements AR v.4.6
- Advanced research package capable of mult-dimensional image acquisition including multiple XY positions, z-series, multiple channels, time and large image stitching
Upright widefield microscope:
- Nikon E-55i
- Training initiated via iLab
- Reservation via shared outlook calendar
- 4-100x objectives mounted
- Brightfield only (no phase/DIC)
- Color and Monochrome cameras mounted
- DS-Fi1 color camera (5M pixel; 12-bit)
- DS-Qi1Mc monochrome camera (1.3 M pixel CCD)
- Mercury Arc Lamp flourescence light source
- DAPI/FITC/Texas Red cubes mounted
- NIS-Elements Documentation (v 5.20.00)
Inverted widefield microscopes:
- Nikon TE-2000
- Training initiated via iLab
- Reservation via shared outlook calendar
- 4-40x phase objectives
- QI Fast 1394 12-bit color camera
- Mercury Arc Lamp fluorescence light source
- DAPI/FITC/TRITC/Texas Red cubes available
- NIS-Elements Basic Research v 5.20.00
- Keyence BZ-X810
- Training initiated via iLab
- Reservation via shared outlook calendar
- All training via Keyence Representative (Contact Andrew Barr for guidance)
- 4-40x phase objectives
- Cubes mounted (4-positions):
- Multiple stage mounts
- slides (mount 3 at a time)
- dishes (35 mm – 10cm)
- multi-well plate
- Keyence acquisition & analysis software
- automated XYZ stitching of large histology sections; screening and quantification of multi-well plates.
Leadership
Andrew Barr | Research Program Coordinator Ill
andrewbarr@mcw.edu
Megan Harwig, Ph.D. | Research Scientist II | Microscopy Core Manager
Location and hours of operation
| Hours |
Location |
|
Monday - Sunday
24/7
|
8701 Water Town Plank Rd
Wauwatosa WI 53226
|
Welcome!
