Overview of CVC Cores
CVC-iPSC Core Services
Human Induced Pluripotent Stem Cell Program
Our Vision:
To be the regional leader in providing quality iPSCs with a matching biorepository for translational and precision medicine research.
Our Mission:
To improve overall health in Southeast Wisconsin and beyond through impactful translational science that improves research and patient care
across all ages by providing culturing services of patient samples into iPSCs, karyotyping and gene-editing, and acting as a biobank of clinical-grade iPSCs.
Services Offered:
- Reprogramming
- Reprogramming of PBMC into iPSCs
- Reprogramming of fibroblasts into iPSCs
- Reprogramming of UDCs into iPSCs
- Differentiation
- Differentiation of iPSCs into 2D- Kidney Organoids
- Differentiation of iPSCs into Cardiomyocytes
- Differentiation of iPSCs into endothelial cells
- Differentiation of iPSCs into Vascular Smooth Muscle Cells
- Karyotyping
- Karyotyping (per Colony) Minimum of 2 Colonies.
- Fibroblast Isolation
- Generation of Urine-Derived Cells (UDCs)
- Induced Pluripotent stem cells (iPSCs)
- PBMC (Peripheral blood mononuclear cells) processing
CVC-Microscopy Core
All users must be members of the CVC. Contact Andrew Barr for guidance on becoming a CVC member.
$25/hr charge for use of the Nikon A1R, all other resources are free to use once trained.
Inverted confocal microscopes:
- Nikon A1R Laser Scanning Confocal ($25/hr)
- Training initiated & reserved via iLab
- 10-100x DIC objectives
- Excitation laser lines 405/488/561/640 nm
- Emission band passes (425-475/500-550/570-620/663-738)
- Galvano or fast resonant scanning
- Live cell imaging chamber: OKO lab boldline temp + CO2/O2 stage top incubator available
- LED light source
- NIS-Elements AR v.5.21.03
- Advanced research package capable of mult-dimensional image acquisition including multiple XY positions, z-series, multiple channels, time and large image stitching
- Nikon’s denoise.ai
- Photobleaching/photo-stimulation module available (Galvano = all lasers available for photobleaching/photostimulation; Resonance = only 405 nm laser available for photobleaching/photostimulation)
- Nikon C2+ Laser Scanning Confocal (no charge)
- Training initiated via iLab
- Reservation via shared outlook calendar
- 20-40x objectives mounted; 60x & 100x available as needed
- Excitation laser lines 405/488/561/640
- Manual 2 detector system allows simultaneous imaging of up to 3 channels (4 channels may be imaged sequentially):
- Position 1: 445/35 nm & 660 nm LP
- Position 2: 525/50 nm & 600/50 nm
- Live cell imaging chamber: OKO lab boldline temp + CO2/O2 stage top incubator available
- Metal halide light source
- NIS-Elements AR v.4.6
- Advanced research package capable of mult-dimensional image acquisition including multiple XY positions, z-series, multiple channels, time and large image stitching
Upright widefield microscope:
- Nikon E-55i
- Training initiated via iLab
- Reservation via shared outlook calendar
- 4-100x objectives mounted
- Brightfield only (no phase/DIC)
- Color and Monochrome cameras mounted
- DS-Fi1 color camera (5M pixel; 12-bit)
- DS-Qi1Mc monochrome camera (1.3 M pixel CCD)
- Mercury Arc Lamp flourescence light source
- DAPI/FITC/Texas Red cubes mounted
- NIS-Elements Documentation (v 5.20.00)
Inverted widefield microscopes:
- Nikon TE-2000
- Training initiated via iLab
- Reservation via shared outlook calendar
- 4-40x phase objectives
- QI Fast 1394 12-bit color camera
- Mercury Arc Lamp fluorescence light source
- DAPI/FITC/TRITC/Texas Red cubes available
- NIS-Elements Basic Research v 5.20.00
- Keyence BZ-X810
- Training initiated via iLab
- Reservation via shared outlook calendar
- All training via Keyence Representative (Contact Andrew Barr for guidance)
- 4-40x phase objectives
- Cubes mounted (4-positions):
- Multiple stage mounts
- slides (mount 3 at a time)
- dishes (35 mm – 10cm)
- multi-well plate
- Keyence acquisition & analysis software
- automated XYZ stitching of large histology sections; screening and quantification of multi-well plates.
Leadership
Andrew Barr | Research Program Coordinator ll
andrewbarr@mcw.edu
Gracious Ross, Ph.D. | Research Scientist II | iPSC Core Manager
gross@mcw.edu
Megan Harwig, Ph.D. | Research Scientist II | Microscopy Core Manager
Location and hours of operation
| Hours |
Location |
|
Monday - Sunday
24/7
|
8701 Water Town Plank Rd
Wauwatosa WI 53226
|
Welcome!
