About

CVRC Confocal Core

For iPSC-related services, please navigate to here

CVRC-Microscopy Core 

All users must be members of the CVC. Contact Andrew Barr for guidance on becoming a CVC member. 

$25/hr charge for use of the Nikon A1R, $20/hr for the use of the Evident VS-200 slide scanner, all other resources are free to use once trained. 

All training provided by Dr. Megan Harwig. Training can be initiated via iLab, ok to directly email to book training sessions.

Be sure to cite RRID:SCR_028523 on your publications that used this Core.

Inverted confocal microscope: 

  • Nikon A1R Laser Scanning Confocal ($25/hr)

    • Nikon Eclipse fully motorized inverted microscope
      • Universal stage holder (slides and dishes)
      • Multi-well plate holder
    • 10-100x DIC objectives
    • Excitation laser lines 405/488/561/640 nm
    • Emission band passes
      • 425-475
      • 500-550
      • 570-620
      • 663-738
    • Galvano or fast resonant scanning
    • Live cell imaging chamber: OKO lab boldline temp + CO2/O2 stage top incubator available
    • LED light source
    • NIS-Elements AR v.6
      • Advanced research package capable of mult-dimensional image acquisition including multiple XY positions, z-series, multiple channels, time and large image stitching
      • Nikon’s denoise.ai
      • Photobleaching/photo-stimulation module available (Galvano = all lasers available for photobleaching/photostimulation; Resonance = only 405 nm laser available for photobleaching/photostimulation)
    • Reserved via iLab calendar

Upright widefield microscopes:

  • Evident VS-200 Slide Scanner with SILA technology ($20/hr)

    • Fully enclosed upright widefield microscope
    • 6-slide holder (1x3 glass slides)
    • Imaging modes: 
      • Brightfield, Polarization, Darkfield, Fluorescence, and “Confocal Like” SILA optical sectioning
    • Objectives ranging from 2x to 100x oil 
      • equipped with an auto oiler for the 40x and 100x oil objectives
    • Two cameras
      • CMOS color camera for brightfield imaging
      • ORCA-Fusion BT sCMOS for fluorescence imaging
    • 2 excitation options:
      • Excelitas X-Cite XYLIS 
        • single pass cube options for the LED (DAPI/EGFP/Cy3/Cy5/Cy7)
      • Laser options for SILA imaging (395, 488, 561 and 638 nm laser)
        • multi-band cube for SILA imaging: (350/470/545/620/710)
    • Software: VS200 software capable of automated whole tissue selection, automated focus map creation and batch capture
    • Reservation via iLab calendar

 

  • Nikon upright manual widefield microscope

    • Color and Monochrome cameras mounted
      • DS-Fi1 color camera (5M pixel; 12-bit)
      • DS-Qi1Mc monochrome camera (1.3 M pixel CCD) 
    • Mercury Arc Lamp flourescence light source
    • DAPI/FITC/Texas Red cubes mounted
    • 4-40x objectives mounted; Brightfield only (no phase/DIC)
    • NIS-Elements Documentation (v 5.20.00)
    • Reservation via shared outlook calendar

 

Inverted widefield microscopes:

  • Nikon C2PO (Formerly known as Nikon C2 Laser Scanning Confocal, now pillaged and overhauled and is a widefield only station)

    • Nikon Eclipse fully motorized inverted microscope
      • Universal stage holder (slides and dishes)
      • Multi-well plate holder
    • 10-100x objectives mounted; mix of phase and brightfield 
    • D-LED individually controlled excitation lines 405/488/561/640
    • DAPI/FITC/TRITC/Cy5 and a Quad band multi-pass cube are available
    • Moment monochrome camera
    • NIS-Elements AR v.6
      • Advanced research package capable of mult-dimensional image acquisition including multiple XY positions, z-series, multiple channels, time and large image stitching
    • Reservation via iLab

 

  • Keyence BZ-X810

    • Fully enclosed and motorized inverted microscope
    • Stage options:
      • slides (mount 3 at a time)
      • dishes (35 mm – 10cm)
      • multi-well plate
    • 4-60x objectives (mix of phase and brightfield only)
    • Cube options (4-positions):
      • DAPI/EGFP/Cy3/mCH/CY5
    • Optional Tokai incubation system for 5% CO2 and 37°C live cell imaging support
      • Can accomodate plates, slides and dishes
    • Keyence acquisition & analysis software
      • automated XYZ stitching of large histology sections
      • automated batch acquisition and analysis (ideal for multi-well plates)
      • Time-lapse imaging
      • Optical sectioning module
    • Reservation via shared outlook calendar

 

  • Nikon TE-2000 

    • Nikon TE-2000 manual inverted microscope
    • DAPI/FITC/TRITC/Texas Red cubes available
    • 4-40x phase objectives
    • Metal halide light source
    • Quick checks only → No camera
    • Walk-up use

 

Analysis Software

 

  • Evident Analysis Station

    • Full verions of VS200 software capable of MaxIP projections and EFI conversions
      • Batch image format converter
      • Detection and analysis (count and measure); See Evident Videos for count and measure guidance
      • 3D deconvolution
    • Open source software also installed: QuPath, Cell Profiler and FIJI

 

Be sure to cite the following in your publications that use these resources: 

Medical College of Wisconsin - Cardiovascular Research Center (CVRC) Microscopy Core, RRID:SCR_028523

Once the RRID is used in a publication is automatically appears on the CVRC CoreMarketplace page: 

The CoreMarketplace: Medical College of Wisconsin - Cardiovascular Research Center (CVRC) Microscopy Core

 

Leadership

Andrew Barr | Research Program Coordinator Ill

andrewbarr@mcw.edu

Megan Harwig, Ph.D. | Research Scientist II | Microscopy Core Manager

 

Location and hours of operation

Hours      Location

Monday - Sunday
24/7 

     8701 Water Town Plank Rd
     Wauwatosa WI 53226

 

Other imaging cores on campus: 

CRI imaging core 

  • Zeiss Confocal Microscope
  • Zeiss Confocal / Multiphoton Microscope
  • Zeiss Upright Microscope
  • Zeiss Inverted Microscope
  • PALM Laser Microdissection
  • Laser Scanning Cytometry
  • Hamamatsu (Brightfield) slide scanning drop off service
  • VS120 (Fluorescence) slide scanning drop off service 
  • Image Analysis Consultation service

NRC Microscopy Core

  • Imaris Image Processing Computer
  • Leica SP8 Upright Confocal Microscope
  • Stereoscope
  • Tissue clearing service
  • Lightsheet Microscopy service
  • Nikon Multiphoton (not available yet as shared resource)

OxCAM-EM core

  • Andor Spinning Disk Confocals
    • BC-43: benchtop inverted spinning disk confocal
    • BC-43: benchtop inverted spinning disk confocal with live cell capabilities
    • Dragonfly 502 inverted spinning disk confocal with Mosaic and micro-injection capabilites
    • Dragonfly 620 SR inverted spinning disk confocal with STORM/TIRF/SRRF capabilities
  • Nikon N-SIM 
  • Abberior Facility Line STED

Structural Biology Shared Resources (SBSR)

  • Thermo Glacios 200 kV cryo-TEM for single particle analysis

Versiti

  • Nikon Ti2 Widefield inverted microscope
  • Nikon CSU-W1 Spinning Disk inverted confocal + SoRa
  • 3i upright spinning disk confocal
  • Olympus Laser Scanning Confocal and Multi-photon
  • EVOS M7000
  • Motic EasyScan Pro6 Digital Slide Scanner
  • Zeiss Lumar Stereo Microscope
  • Analysis stations
    • Nikon NIS-Elements
    • Slidebook
    • Imaris - East
    • Imaris - West
  • Nikon AxR (not available yet as shared resource)
  • Nikon Multiphoton (not available yet as shared resource)

Contacts

Name Role Phone Email Location
Andrew Barr
Research Program Coordinator Ill
 

 
andrewbarr@mcw.edu
 
8701 Water Town Plank Rd, Wauwatosa WI 53226
 

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