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MCW Tissue Bank

Overview & Services

The MCW Tissue Bank is an institutional core that provides research services involving human samples to faculty investigators.  The Tissue Bank is a CAP-accredited biorepository, which ensures high-quality samples, vetted protocols and standard operating procedures, and knowledgeable, well-trained staff.  The Bank offers robust consent programs to patients of Froedtert Hospitals and clinics, including the General Tissue Bank, the Maternal Bank, the COVID-19 Bank.  In addition to providing tissues and other biospecimens to researchers, the Bank also provides a wealth of services associated with human samples; storage and processing among many.

 

Biofluids 

  • Services: 
    • Buffy coat lysis 
    • PBMC processing 
    • Simple blood processing 
    • Cell counting 
  • Samples: 
    • Whole blood – fresh or frozen 
    • Plasma – fresh or frozen 
    • Buffy coat – fresh or frozen 
    • Cord blood – fresh 
    • Plasma from cord blood – fresh or frozen 
    • Buffy coat from cord blood – fresh or frozen 
    • PBMCs 
    • Serum from recovered COVID-19 patients – fresh or frozen 
    • Fresh bone marrow
    • Cryopreserved, viable bone marrow MNCs
    • Bone Marrow MNCs (lysed discard samples from flow cytometry) – fresh or frozen 

Tissue

  • Services:
    • Fresh tissue procurement (e.g. tumor, cardiovascular, maternal tissues)
    • Snap freezing
  • Samples:
    • Fresh tissue (e.g. for PDX modeling, ex vivo explants or organoids, cell lines)
    • Snap frozen tissue
    • Formalin fixed paraffin embedded (FFPE) tissue
    • Frozen tissue shaves

We perform Quality Control on banked samples

Tissue Arraying  Services

  • Tissue Array Construction:
    • Standard core-based tissue microarrays (TMA)
    • Tissue arrays by Cutting-Edge Matrix Assembly (CEMA)
    • Sausage arrays for use as positive and negative controls
    • Cell pellet arrays

Multi-color Immunohistochemistry and Histocytometry analyses

  • Tumor Multicolor quantitative immunofluorescence histo-cytometry (7-color Vectra Polaris)
    • Solid tumor immunoprofiling (CD4, CD8, CD20, FoxP3, CD68, pan-Cytokeratin, DAPI)
    • Custom 7-color multiplex immunostaining panels

Storage, supplies, and other services

  • Data logging and barcoded labeling of samples
  • Media, storage containers, and laboratory supplies
  • Monitored, secure -80°C storage
  • Monitored, secure LN/-150°C cryostorage

 

Leadership

Hallgeir Rui, MD PhD | Director

Mary Rau | Manager

 

Location and hours of operation

Hours Location

Monday - Friday 8 a.m. - 5 p.m.

(excluding MCW holidays)                   

9200 W. Wisconsin Avenue

Milwaukee, WI 53226

Website

Contacts

Name Role Phone Email Location
Mary Rau
Tissue Bank Manager
 
(414) 805-9569
 
mrau@mcw.edu
 
MCW Tissue Bank
 
Mollie Patton
Pathologists' Assistant
 
(414) 805-8823
 
mpatton@mcw.edu
 
MCW Tissue Bank
 
Jasmine Pickett
Assistant Pathologists' Assistant
 
(414) 805-8824
 
jpickett@mcw.edu
 
MCW Tissue Bank
 
Ellen Schneider
Research Technologist III
 
(414) 805-9587
 
eschneider@mcw.edu
 
MCW Tissue Bank
 
MCW Tissue Bank
Lab All-Staff Phone
 
(414) 805-8829
 
mcwtissuebank@mcw.edu
 
MCW Tissue Bank
 
Kelly Putnam
Histology Technician
 
(414) 805-8821
 
kputnam@mcw.edu
 
MCW Tissue Bank
 
Kayla Schwartz
Research Technologist I
 
(414) 805-8822
 
kschwartz@mcw.edu
 
MCW Tissue Bank
 
Gwenyth Gasper
Clinical Research Assistant II
 
(414) 805-9767
 
ggasper@mcw.edu
 
Pathology Administration Suite
 
Jamie Pipke
Clinical Research Assistant II
 
(414) 805-6588
 
jpipke@mcw.edu
 
Pathology Administration Suite