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Childrens Research Institute Histology Core

  

 

         

Overview of Services

 

The Children’s Research Institute (CRI) Histology Core offers a full range of routine and specialized histological, immunological and related services on a fee-for-service basis for principle investigators from Children’s Hospital of Wisconsin, the Medical College of Wisconsin and other outside institutions. We have worked with over 170 investigators on countless projects!  This full service Histology Core facility is operated in conjunction with the Pediatric Pathology Division of the Department of Pathology, and is under the scientific direction of board certified Pathologists. 

 

ROUTINE Services

  • Tissue Grossing and Processing
  • Embedding: Paraffin & OCT (fresh, fixed, frozen tissue) with attention to orientation
  • Sectioning: Paraffin, OCT (frozen 2-50μm), Vibratome (50-2000μm)
  • Automated H&E staining
  • Special Stains:  Pigment and Mineral, Microorganism, Carbohydrate, Nerve, and Connective Tissue
  • Immunohistochemical (IHC) & Immunofluorescent (IF) staining
  • Optimization of user-provided antibodies for IHC and IF
  • Free Floating IF for thick sections
  • Controls with all approved stains
  • Cytospin preparation

CUSTOM Services

  • Specialized Histology for a variety of human, animal, insect, fish and insect species
  • Paraffin preparation for cells and organoids
  • Development and optimization of additional special stains
  • Preparation and staining of tissue sections for Laser Capture Microdissection
  • Small-group discussions

Leadership

Jason Jarzembowski, MD PhD | Director

Paula North, MD PhD | Scientific Director

Andrew Webb | Division Administrator

Christine Duris, BS HTL QIHC (ASCP)cm | Supervisor

 

Histology Core Staff

Qiuhui Yang, MD PhD HTL(ASCP)cm

Tanya Bufford HT(ASCP)

Daraphone Zieske, BS HTL (ASCP)

 

Location and Hours of Operation

Hours Location

Monday - Friday

8am - 3:30pm                 

8701 Watertown Plank Road, C4305 CRI-TBRC

Milwaukee, WI 53226

Links and Resources

Contacts

Name Role Phone Email Location
Christine Duris, BS HTL QIHC (ASCP)
Supervisor
 
414-955-8624
 
cduris@mcw.edu
 
C4305 CRI-TBRC
 
Tanya Bufford, HT (ASCP)
Histotechnologist
 
414-955-8624
 
tbufford@mcw.edu
 
C4305 CRI-TBRC
 
Qiuhui Yang, MD PhD HTL(ASCP)
Research Scientist
 
414-955-8624
 
qyang@mcw.edu
 
C4305 CRI-TBRC
 
Daraphone Zieske, BS HTL (ASCP)
Histotechnologist
 
414-955-8624
 
dzieske@mcw.edu
 
C4305 CRI-TBRC