Mouse Transgenic Core

Overview of Services

The Mouse Transgenic Core assists research staff with procedures involving production and maintenance of transgenic mouse strains.  The Core provides both sperm and embryo cryopreservation/recovery, IVF, pronuclear microinjection of CRISPR and DNA/BAC constructs.  We are also available for consultation involving colony breeding issues.  

The laboratory is located in the vivarium of the Translational and Biomedical Research Center at MCW and is equipped to perform all procedures involving production and maintenance of transgenic or knock-out mice. Instrumentation includes 2 injection workstations (Nikon inverted phase contrast microscope with Hoffman modulation optics, temperature-controlled injection stage, air-suspended injection table, Eppendorf motor-driven injection system), microforge, needle puller, Nikon stereo zoom microscope, surgical microscopes and dewars for storage of cryopreserved mouse germplasm.  

Leadership

Aron Geurts, PhD

Location and hours of operation

Links and Resources

1. [GSPMC transgenic core website]
2. [ link or resource # 2 ]
3. [ etc... ]

Contacts