Cancer Center Redox and Bioenergetics Shared Resource (CCRBSR)

Overview of Services

The MCW Cancer Center Bioenergetics Shared Resource (CCBSR) is dedicated to cancer research by utilizing cutting edge technology to make advancements in the field. The MCW CCBSR is located on the second floor in the MACC Fund Research Center (MFRC), Room 2013. Two main instruments are housed within the CCBSR laboratory: a Shimadzu Liquid Chromatography / Mass Spectrometry 8030 System (Shimadzu LC MS/MS (Fig. 2A) and  Seahorse XF96 and XF^e96 extracellular flux analyzers (Agilent Technologies) (Fig. 2B). These instruments enable profiling and quantification of multiple metabolic pathways, including assessment of mitochondrial and glycolytic function in cancer cell culture models, allow for assessment of mitochondrial toxicity of novel chemotherapeutic agents, and can be used in longitudinal studies of tumorigenesis prevention. The Shimadzu LC MS/MS system is also capable of detecting and quantifying multiple metabolites simultaneously in biological samples. Investigators wishing to use these instruments design experiments in collaboration with the MCW CCBSR staff to ensure technical proficiency and accurate analysis of the data.

Services Provided: A wide range of experimental applications of extracellular flux and metabolomic studies can be performed with the instruments in the MCW CCBSR facility. Using the Seahorse XF96 and XF^e96 (a fluorescence-based microplate assay), we can simultaneously measure both oxygen consumption rate and extracellular acidification rate in a noninvasive manner. The Seahorse XF96 and XF^e96 systems allow investigators to examine mitochondrial function, glycolytic function, fatty acid oxidation, glucose metabolism, glutamine metabolism, and metabolic phenotypes all in cancer models. Using the Shimadzu LC MS/MS, we offer enhanced and fast detection and quantification of extra- and intra-cellular substrates and metabolites, metabolic profiling/phenotyping, and drug/compound screening. The instruments are dedicated solely to analyze cancer bioenergetics, making the MCW CCBSR not only an important resource, but also extremely unique, since both instruments are not available elsewhere on the MCW campus.

Leadership

Balaraman Kalyanaraman, PhD | Director

Jacek Zielonka, PhD | Research Director

Location and hours of operation

Links and Resources

• https://www.mcw.edu/Research-Resources/Cores/Cores-and-Shared-Resources/Bioenergetics-Shared-Resource.htm

Contacts